1) SpectraMax Glo Steady Luc Reporter Assay (6. The REF button has no valid meaning for fluorometric use, and if pressed when in fluorometric mode, will switch the mode to absorbance cuvette (thus the emission wavelength display disappears). com. VersaMax ELISA. book Page 1 Wednesday, July 7, 2010 2:09 PM. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. 7°C and the bottom to 36. Monochromator, tunable 1. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. Operated fromTo ensure loading of equal amounts of protein for each sample, colorimetric protein quantification was performed using a Bio-Rad Protein Assay (Bio-Rad, Hercules, CA) and a SpectraMax M3 spectrophotometer (Molecular Devices, Sunnyvale, CA). 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax Microplate Stacker for walk-away processing. Products : SpectraMax M3 reader, SpectraMax M4 reader, LMax II (Obsolete), FlexStation 3 reader, SpectraMax L reader, SpectraMax M5/M5e reader How do I know if the LM-1 plate is off or on? The LM1 plate has a push button switch in a cupped-out area on one end. SpectraMax Microplate Readers. If no sound is heard during initialization, contact technical support. spr SpectraMax iD3 SpectraTest ABS2 v1. The test was repeated three times. Remove the transport bracket and store the retaining screws in the bag that held the 3. ® reader to the computer. FlexStation™ I. Find regional support. The SpectraMax M3 microplate reader (Molecular Devices, USA) was used to monitor the colour development over 5-min intervals for 30 min at 370 nm. All procedures completely complied with the manufacturer’s instructions. For more detailed information and technical support of Life Technologies assays including specific conditions for assay windows between 2-3 fold, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Shake intensity. Sphere-like cells were subcultured into single-cell suspension, and cells of ≥90% viability were used for sphere formation studies. Add to Cart. The resulting absorbance values were plotted against time. In this application note, we demonstrate the measurement of luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals. 标准功. Table 1. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellLuminescence is the emission of light by a substance as a result of a chemical reaction called chemiluminescence or an enzymatic reaction called bioluminescence. 4/5 Set up plate template / Define Plate Map 1. Molecular Devices SpectraMax M3 User Manual (86 pages) Multi-Mode Microplate Readers. The detection of intracellular ROS and mitochondrial membrane potential. The fluorescence intensity of the cells was detected using a SpectraMax M3 florescence microplate reader (Molecular Devices) with excitation and emission wavelengths of 485 and 535 nm, respectively. In addition, the total flavonoid content was determined using the aluminum chloride method. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 4. Qualify the fluorescence performance of the SpectraMax . It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely low light. SpectraMax M3 Multi-Mode Microplate Reader. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. SpectraMax Mini. The SpectraTest ABS1 Validation Plate is a comprehensive optical validation package that is com-pletely automated when used with SoftMax ® Pro software. D8000C; R&D Systems, Minneapolis,. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. No accessories are needed and SoftMax® Pro. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. 4. book Page 1 Tuesday,. The optical density was read using a SpectraMax M3 (Molecular Devices, Sunnyvale, CA, USA). INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThe SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. 313 reference gain check fail. Microplate Reader Shelf. reader in the visible range between 405–750 nm with NIST and NMI. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Align the two slots on the bottle holder with the two pegs on the rail. Then, adding 2 M sulfuric acid, the reaction was stopped, and the absorbance at 450 nm was determined using a SpectraMax M3 microplate reader (Molecular. 0 out of 5 $ 11,999. 190-1000 nm, tunable in 1 nm increments. SoftMax Pro GxP is a special software release that includes full user and permission con-SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. 発光モードに対応した機種（SpectraMax M5e/M5/M4/M3）では、発光モード専用のフォトンカウント検出器も搭載しました。 蛍光に特化した検出器を流用するのではなく、専用の光学系を採用することで、発光、TR. After that, 100 µL of TMB solution was added as the substrate and kept for 15 min at room temperature. 4. 3 software (Molecular Devices, San Jose, CA, USA). Reference and the Water Constant methods. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Microtitre plates were incubated for 2 h at 37°C in the dark. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Amplitude : 1. 0. Lift the right side of the microplate reader and place the feet into the two mounting cups on the baseplate. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Also, when using a SpectraMax M4 Reader,. Photometric accuracy. Description 1. For a complete list of the instruments supported by this release of the8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. SpectraMax M5/M5e/M4/M3/M2/M2e. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. . The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell. Data were expressed as mg/g gallic acid equivalents (GAE) of dry crude extract. Data were analyzed using Parallel Line Analysis 2. In addition, they allow to increase their performance sequentially from models: M2 ‘ M2e;. Other technologies such as imaging. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 0 mm hex key. 314 low lamp level warning. STAKMAX. Molecular Devices’ unique SpectraDrop™ MicroVolume Microplate offers the highest throughput solution for low volume measurement available on the market today. Microplate Readers Tecan Infinite M200 Plate Reader. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Recommended SpectraMax iD3/iD5 reader settings. 4. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. The drawing shows a standard microplate of 15 mm height covered with a lid. This process should take about 3 - 5 minutes. Plate Formats: 6, 12, 24, 48, 96, 384 wells. SpectraMaxi3Multi. com. Add cells in appropriate medium to microplate wells. UV Qualification, using NIST traceable standards, is now available for SpectraMax 190, Plus 384 and the M Series (M2/M3/M4/M5/M5e) Parameters that are checked during the UV Qualification are: Wavelength accuracy. Table of Contents. Guanidine hydrochloride at a final concentration of 5 M was used to denature a 10 mg/mL solution of lysozyme. Click template editor icon in Home panel or select configure your plate layout in plate setup helper a. . Molecular Devices LLC spectramax m3 multi mode microplate reader spectrophotometer Spectramax M3 Multi Mode Microplate Reader Spectrophotometer, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. SpectraMax ® M2 Multi-Mode Microplate Reader. BioTek Synergy LX Multi-Mode Microplate Reader (FLD/Lumi) Accuris MR9600 SmartReader 96 and MW9600 Smartwasher 96 Bundle, 115V. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected] i3x Multi-Mode Detection Platform Features User-configurable applications User-configurable detection modules expand the reader’s detection capabilities to. The concentration of CTC was expressed as mg catechin equivalent per g of extract. 2. All associated StakMax® stackers. book Page 1 Wednesday, July 7, 2010 2:09 PM. Bioz Stars score: 86/100, based on 1 PubMed citations. Figure 1. I am looking to meet/connect with people from all walks of life. The entire SpectraMax M range includes a cuvette port for Absorbance, Fluorescence, and from SpectraMax M3 also for Luminescence. Data of selected analytes in the graphs below illustrate sample correlations between 96-well and 384-well format of 16 human plasma samples. Filterless Absorbance Microplate Readers provides fast & consistent results with steady temperature enabling a wide range of assays - ELISA reader, microbial growth, & protein quantitation. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. The assay was repeated at varying final concentrations (5, 15, 25, 50, 100 nM) of PES. The chamber contains springs that position the cuvette in the proper alignment for a read. Louis, USA). The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. 1. Page 1 SpectraMax ® GEMINI EM Dual-Scanning Microplate Spectrofluorometer Operator’s Manual 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0088 Rev. ShakerTime: 0 to 999 seconds. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precisionAnalytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). Total phenolic contents were calculated from the gallic acid standard curve. Add to Cart. 0 out of 5 $ 2,999. The absorbance was assessed at 405 nm by a SpectraMax M3 microplate reader (Molecular Devices, Sunnyvale, CA, USA). no. SpectraMax ABS Plus SpectraTest ABS2 v1. I used the same plate and set the PMT to be automatic. Unplug the power cable from the back of the instrument. All configurations. Choose from a three- (M3), four- (M4), orThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. 0200-6117. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. When a feature or capability applies to only certain readers, this exception is noted. Page 22 SpectraMax L Luminescence Microplate Reader User Guide To manually change the aperture: 1. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Danaher Inc molecular devices spectramax m3 spectrophotometer Molecular Devices Spectramax M3 Spectrophotometer, supplied by Danaher Inc, used in various techniques. The relative number of viable cells was quantified by measuring the fluorescence (excitation at 560 nm and emission at 590 nm) using a SpectraMax ® M3 (Molecular Devices, San Jose, CA, USA). Dead Time Correction accounts for the finite amount of time it takes the instrument to detect and then record the absorbance and counting of a photon by the. The SpectraMax Multi-Mode Microplate Reader offers both the Cuvette. Extraction of EPS. The corrected absorbance is within 5% of the. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Luciferase Detection Instrumentation Guide. no. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. 0nm increments. The spectrophotometer (SpectraMax, M3, Molecular Devices, Washington DC, USA) was used to confirm the encapsulation of gallic acid inside the PLGA nanoparticles. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. 312 gain calibration failed. ZERO BIAS - scores, article reviews, protocol conditions and moreInstruments and Settings for Fluorescence Read-out (Other Fluorescent Assays) FLUOstar Omega, VANTAstar, CLARIOstar, PHERAstar, PolarStar Optima. Unparalleled performance on a personalized platform. {"product":{"total":4,"offset":0,"limit":4,"data":[{"path":"/products/microplate-readers/multi-mode-readers/spectramax-m2-m2e-readers","identifier":"SpectraMax M2/M2e. Page 13 Installing and Setting Up the. Then, 25 nM HOPS complex, 50 nM Sec18, 600 nM Sec17, and finally 100 nM Vam7 were added. Finally, SpectraMax M3 microplate reader was used to detect 412 nm absorption values, which were positively correlated with GSH levels. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax ABS Plus SpectraTest ABS2 v1. Moffitt Cancer Center. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. 1. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. D 1 1. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M3. Also refer to this Multimode Reader Comparison Chart. . The use of gene reporters such as luciferase permits highly sensitive and nondestructive monitoring of gene expression. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). When used with SoftMax® Pro Data Acquisition and Analysis Software, it can automatically normalize the well absorbance to a cuvette equivalentThe SpectraMax iD3 reader offers the sensitivity and wavelength scanning capability needed to perform intrinsic tryptophan fluorescence measurements. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. The M4 Reader offers the same modes as the M3 Reader, plus time-resolved fluorescence,. The instrument uses a paired monochromator and xenon flash lamp for absorbance reads allowing for optimization of wavelengths between 200 nm and 1000 nm. AquaMax® 4000. 과 같은 Parameter를 검사하여 평가할 수 있습니다. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. , 2020). 1. Finally, the absorbance was measured with a SpectraMax M3 microplate reader (Molecular Devices Corporation, Sunnyvale, CA, USA) at a wavelength of 450 nm. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. When a feature or capability applies to only certain readers, this exception is noted. The SpectraMax ABS Plus reader utilizes Molecular . 2. Cables. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 7°C and the bottom to 36. Italic and boldface type are used for emphasis. I don’t care what you do or where…The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a series of multi-detection, dual-monochromator, multi-mode instruments with a triple-mode cuvette port and 6 to 384-well microplate reading capabilities. Description 1. Microplate Readers Molecular Devices VersaMax Microplate Reader. Operated from within SoftMax Pro Software, the StakMax Microplate Handler can hold up to 50 plates and facilitates barcode reading. Italic and boldface type are used for emphasis. 9000-0756. Abstract. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. 2. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and. For more sample throughput, the system can StakMax Handling Stacker Base Unit. 90-0083 series): Excitation 530 nm,. Resolution: In the GxP audit trail, the SpectraMax M3 and M4 Readers are now identified correctly. RP-3500 is a novel, orally bioavailable clinical. edu, 814-863-3503. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. a SpectraMax M3 microplate reader (Molecular Devices Inc. LINKS to Craig…His YouTube is @BrickArmy and his business is: is COBI’s website: set in this video is #2535 M. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. The innovative and flexible design features. 0112-0112F 3 Contents Chapter1:SpectraTestABS1ValidationPlateOverview 5 CertificateofCalibration 6 ISO-17025 6 AvailableTests 7 Chapter2:ValidationProtocols 9 SpectraMax® L (software version 5. U2OS cell viability (%) = [A cells with different treatments −A contro ]/[A cells with no treatments − A control ] × 100. SpectraMax M3 Multi-Mode Microplate Reader. Ataxia telangiectasia and Rad3-related (ATR) kinase protects genome integrity during DNA replication. . 1 Defect ID: FB 2570 These two validation protocols are not included in the v5. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax® iD3 Multi Mode Microplate . Page 26 The trademarks used herein are the. The reaction was terminated with 100 µL of dinitrosalicylic acid and placed in a boiling water bath for 5 min. Metrics used to measure sensitivity include signal-to-background ratio (S/B), signal-to-noise ratio (S/N), lower limit of detection (LLD) and Z’-factor 1. We would like to show you a description here but the site won’t allow us. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-Mode Note: In this user guide, SpectraMax ® refers to several SpectraMax . Shop For Laboratory Equipment featuring a wide variety of new, used and refurbished laboratory equipment. The Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is a multi-detection microplate reader with a dual-mode cuvette port and 6-384 microplate reading capability. Learn more about its features, specifications, and applications in this user guide. Note: you might need to slide the microplate reader on the foam pad to make room for the cytometer. 1. The ACE2 activity assay was performed in black 96-well plates with 50 μM ACE2-specific fluorogenic peptide substrate (M-2660) in a final. the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. 312 gain calibration failed. Description1 Introduction The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. ScanLater Western Blot Detection Cartridge. Molecular Devices LLC pro 6 3 software controlled spectramax m3 multimode microplate reader Pro 6 3 Software Controlled Spectramax M3 Multimode Microplate Reader, supplied by Molecular Devices LLC, used in various techniques. On the front panel of the instrument, press the load/eject button to move the microplate drawer outside of the instrument. SpectraMax® iD5. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. Multi-Mode Microplate Readers. This user guide provides detailed instructions on how to perform various tests, interpret the results, and troubleshoot common issues. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Fluorescence was quantified on SpectraMax M3 plate reader, Ex 485/Em 520 using SoftMax Pro software. D 1 1. Make sure that the microplate reader feet are completely seated in the mounting cups. Twenty five μL of supernatant was removed from each sample and placed in a 96-well clear bottom plate and read at 546/575 nm (excitation/emission) using a SpectraMax M3 fluorescent microplate reader (Molecular Devices, Sunnyvale, CA). The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. 100 µL of MycoAlert Substrate (reconstituted in MycoAlert Buffer) was immediately added to each well and the plate incubated at room temperature for 10 minutes. The net area under the curve (AUC) was estimated by subtracting the AUC of the blank from the AUC of the reacted. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Superior flexibilityThe SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Multimode Reader Comparison Chart. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. The following document is intended to demonstrate setup of this instrument. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid-すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Lift up the lid over the chamber to insert or remove a cuvette. Multimode Reader Comparison Chart. Bioz Stars score: 96/100, based on 1 PubMed citations. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Polyplex Size and Zeta Potential Measurement. Acarbose was used as a. Instructions for using SIRT1 siRNA and scrambled siRNA were provided by Santa Cruz Biotechnology (CA, USA). Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 1step assay with SpectraMax microplate readers. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate ReaderMolecular Devices Spectramax M3 Multi Mode Microplate Reader; Related products. We would like to show you a description here but the site won’t allow us. 2 release. book Page 21 Wednesday, July 7, 2010 2:09 PM. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. The positive control was treated with fresh RPMI-1640 (Lonza, Walkersville, MD). for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 1). Figure 6: Transcreener ADP2 FI assay principle. Then aluminum. 4. Supplier: Molecular Devices. For performing enzyme assays SpectraMax M3 Multi-Mode Microplate Reader (Molecular device, USA) was used. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Background signal was determined by adding 500 U/ml Catalase from Bovine Liver (Sigma-Aldrich, E3289) in one well to convert hydrogen peroxide into. ATP standard curves read on SpectraMax microplate readers. The % parasite viability, as indicated by the pLDH activity in the treated wells compared with the untreated controls, was calculated. The bacterial colony was mixed with. After cooling to room temperature (22 °C), the mixture was transferred to 96-well plates. . SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. 7°C (gradient heating design). Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. 0 out of 5Growth curves were read in a Spectramax M3 plate reader, at 37 °C with shaking at an optical density of 600 nm; reads were taken every 5 min. 2. In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. S. Table of Contents. When a SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Figure 2-23: Bottle holder To install the bottle holder: 1. 2. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. A BCIP/NBT ALP staining kit (Beyotime Institute of Biotechnology, Shanghai, China) was utilized to perform ALP staining on the 7th day according to the manufacturer's instructions. BioTek Synergy 2 SL Luminescence Microplate Reader with PC & Gen5 Software. In the M2, M5 and Plus 384, the light. The software allows custom calculations and personalized reporting due to its analysis, management capabilities, and data acquisitions. The actual pathlength, d, of a solvent is found from the following equation: d cm. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. Results. SpectraMax Spectrofluorometers. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization Options in the instrument settings in SoftMax Pro Software. book Page 1 Wednesday, July 7, 2010 2:09 PM FlexStation® 3 Benchtop Multi-Mode Microplate Reader User Guide 0112-0127 B June 2013 FlexStation_manual. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. ISM Equipment Classification (Group 1, Class A) This equipment is. book Page 1 Wednesday, July 7, 2010 2:09 PM When using a SpectraMax M3 Reader, the GxP audit trail incorrectly reported the instrument as a SpectraMax M4 Read er. Fluorescent Polarization: Solid black plates, as above. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politics光验证板，用于验证 SpectraMax M3、M4 和 M5/M5e 多功能读板机的光 学性能表现。 SoftMax Pro 内置 160 多种预存实验方法可以直接调用，可以进行数据获取和个性化的数据分析；强大的图标绘制工具和文本编辑功能，可以方便的进行各种数 据结果的展示。Popular answers (1) Sanjay Premi. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Supplier: Molecular Devices. SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. SpectraMax® M3. Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader. Plate chamber design of the SpectraMax category 1 microplate readers. 2. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 304 signal level saturation. FI assay. Category 2 and 3 microplate readers use gradient heating as well. Multimode Readers Comparison Chart. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). Read mode. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. 5065390D 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 ComputerIntegration 8 PlateControls 9 CuvetteChamber 10 The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The SpectraMax M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for. Choose cables that meet the requirement described in this appendix. Call 516-939-0888 to check current.